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    TaKaRa agei ecori restriction sites
    Agei Ecori Restriction Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15974 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori+restriction+sites/EcoR+I/pm42014675-126-13-11
    Average 99 stars, based on 15974 article reviews
    agei ecori restriction sites - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: .. Quantification of CD122 association with CD25-CCR7 complex Jo urn al Pr e-p roo f 17 Flag-tagged human CCR7 was PCR-amplified and cloned into the BamHI and EcoRI restriction sites of the 290 lentiviral pLVX-Het1 vector (635075, Takara Bio). .. The resulting plasmid was transfected into HEK293T cells using polyethylenimine (24765, Polysciences), followed by a 48-hour incubation.

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning.
    Article Snippet: Maspardin/SPG21 is a RAB7 effector https://doi.org/10.1083/jcb.202501135 D ow nloaded from http://rupress.org/jcb/article-pdf/225/2/e202501135/1955100/jcb_202501135.pdf by guest on 20 D ecem ber 2025 In-Fusion primers (primer sequences provided in Table S1) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in Table S1), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His (NM_144997.7), pcDNA3.1 TBC1D5HA (NM_014744.2), and pcDNA3.1 GFP-TFEB (NM_007162.2) were bought from GenScript. .. The following plasmids were obtained from Addgene (https://www.addgene.org/): pRK5 Flag-FNIP2 (RRID:Addgene_ 72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) (Tsun et al., 2013); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) (Falcón-Pérez et al., 2005); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_ 99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) (Shen et al., 2017); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) (Mainou and Dermody, 2012); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) (Romero Rosales et al., 2009).

    Article Title: A CD25–chemokine receptor complex initiates noncanonical IL-2 signaling
    Article Snippet: .. FLAG-tagged human CCR7 was PCR amplified and cloned into the BamHI and EcoRI restriction sites of the lentiviral pLVX-Het1 vector (Takara Bio; catalog no.: 635075). .. The resulting plasmid was transfected into HEK293T cells using polyethyleneimine (Polysciences; catalog no.: 24765), followed by a 48-h incubation.

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning
    Article Snippet: The SPG21 sequence was amplified by PCR using In-Fusion primers (primer sequences provided in ) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in ), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His ( NM_144997.7 ), pcDNA3.1 TBC1D5-HA ( NM_014744.2 ), and pcDNA3.1 GFP-TFEB ( NM_007162.2 ) were bought from GenScript. .. The following plasmids were obtained from Addgene ( https://www.addgene.org/ ): pRK5 Flag-FNIP2 (RRID:Addgene_72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) ( ); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) ( ); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) ( ); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) ( ); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) ( ).

    Clone Assay:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: .. Quantification of CD122 association with CD25-CCR7 complex Jo urn al Pr e-p roo f 17 Flag-tagged human CCR7 was PCR-amplified and cloned into the BamHI and EcoRI restriction sites of the 290 lentiviral pLVX-Het1 vector (635075, Takara Bio). .. The resulting plasmid was transfected into HEK293T cells using polyethylenimine (24765, Polysciences), followed by a 48-hour incubation.

    Article Title: NEAT1-mediated regulation of proteostasis and mRNA localization impacts autophagy dysregulation in Rett syndrome
    Article Snippet: .. Two distinct shRNAs (shRNA1 and shRNA2, ) were cloned into the BamHI and EcoRI restriction sites of the pLVX-shRNA2 vector (Cat# 632179, Clontech). .. Two distinct shRNAs (shRNA1 and shRNA2, ) were cloned into the BamHI and EcoRI restriction sites of the pLVX-shRNA2 vector (Cat# 632179, Clontech).

    Article Title: A CD25–chemokine receptor complex initiates noncanonical IL-2 signaling
    Article Snippet: .. FLAG-tagged human CCR7 was PCR amplified and cloned into the BamHI and EcoRI restriction sites of the lentiviral pLVX-Het1 vector (Takara Bio; catalog no.: 635075). .. The resulting plasmid was transfected into HEK293T cells using polyethyleneimine (Polysciences; catalog no.: 24765), followed by a 48-h incubation.

    Plasmid Preparation:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: .. Quantification of CD122 association with CD25-CCR7 complex Jo urn al Pr e-p roo f 17 Flag-tagged human CCR7 was PCR-amplified and cloned into the BamHI and EcoRI restriction sites of the 290 lentiviral pLVX-Het1 vector (635075, Takara Bio). .. The resulting plasmid was transfected into HEK293T cells using polyethylenimine (24765, Polysciences), followed by a 48-hour incubation.

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning.
    Article Snippet: Maspardin/SPG21 is a RAB7 effector https://doi.org/10.1083/jcb.202501135 D ow nloaded from http://rupress.org/jcb/article-pdf/225/2/e202501135/1955100/jcb_202501135.pdf by guest on 20 D ecem ber 2025 In-Fusion primers (primer sequences provided in Table S1) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in Table S1), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His (NM_144997.7), pcDNA3.1 TBC1D5HA (NM_014744.2), and pcDNA3.1 GFP-TFEB (NM_007162.2) were bought from GenScript. .. The following plasmids were obtained from Addgene (https://www.addgene.org/): pRK5 Flag-FNIP2 (RRID:Addgene_ 72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) (Tsun et al., 2013); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) (Falcón-Pérez et al., 2005); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_ 99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) (Shen et al., 2017); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) (Mainou and Dermody, 2012); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) (Romero Rosales et al., 2009).

    Article Title: Multiple interactions recruit BLTP2 to ER-PM contacts to control plasma membrane dynamics
    Article Snippet: .. The full BLTP2^EGFP or BLTP2^Halo sequence was further amplified and inserted between the XbaI and EcoRI restriction sites in a pCAG vector using the In-Fusion system (Takara), respectively. ..

    Article Title: Multiple interactions recruit BLTP2 to ER-PM contacts to control plasma membrane dynamics.
    Article Snippet: .. BLTP2 interactions at ER-PM contacts https://doi.org/10.1083/jcb.202504027 D ow nloaded from http://rupress.org/jcb/article-pdf/224/11/e202504027/1949818/jcb_202504027.pdf by Indian Institute O f Technology Bom bay user on 07 Septem ber 2025 was further amplified and inserted between the XbaI and EcoRI restriction sites in a pCAG vector using the In-Fusion system (Takara), respectively. ..

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning
    Article Snippet: The SPG21 sequence was amplified by PCR using In-Fusion primers (primer sequences provided in ) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in ), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His ( NM_144997.7 ), pcDNA3.1 TBC1D5-HA ( NM_014744.2 ), and pcDNA3.1 GFP-TFEB ( NM_007162.2 ) were bought from GenScript. .. The following plasmids were obtained from Addgene ( https://www.addgene.org/ ): pRK5 Flag-FNIP2 (RRID:Addgene_72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) ( ); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) ( ); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) ( ); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) ( ); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) ( ).

    Sequencing:

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning.
    Article Snippet: Maspardin/SPG21 is a RAB7 effector https://doi.org/10.1083/jcb.202501135 D ow nloaded from http://rupress.org/jcb/article-pdf/225/2/e202501135/1955100/jcb_202501135.pdf by guest on 20 D ecem ber 2025 In-Fusion primers (primer sequences provided in Table S1) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in Table S1), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His (NM_144997.7), pcDNA3.1 TBC1D5HA (NM_014744.2), and pcDNA3.1 GFP-TFEB (NM_007162.2) were bought from GenScript. .. The following plasmids were obtained from Addgene (https://www.addgene.org/): pRK5 Flag-FNIP2 (RRID:Addgene_ 72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) (Tsun et al., 2013); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) (Falcón-Pérez et al., 2005); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_ 99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) (Shen et al., 2017); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) (Mainou and Dermody, 2012); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) (Romero Rosales et al., 2009).

    Article Title: Multiple interactions recruit BLTP2 to ER-PM contacts to control plasma membrane dynamics
    Article Snippet: .. The full BLTP2^EGFP or BLTP2^Halo sequence was further amplified and inserted between the XbaI and EcoRI restriction sites in a pCAG vector using the In-Fusion system (Takara), respectively. ..

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning
    Article Snippet: The SPG21 sequence was amplified by PCR using In-Fusion primers (primer sequences provided in ) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in ), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His ( NM_144997.7 ), pcDNA3.1 TBC1D5-HA ( NM_014744.2 ), and pcDNA3.1 GFP-TFEB ( NM_007162.2 ) were bought from GenScript. .. The following plasmids were obtained from Addgene ( https://www.addgene.org/ ): pRK5 Flag-FNIP2 (RRID:Addgene_72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) ( ); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) ( ); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) ( ); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) ( ); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) ( ).

    Cloning:

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning.
    Article Snippet: Maspardin/SPG21 is a RAB7 effector https://doi.org/10.1083/jcb.202501135 D ow nloaded from http://rupress.org/jcb/article-pdf/225/2/e202501135/1955100/jcb_202501135.pdf by guest on 20 D ecem ber 2025 In-Fusion primers (primer sequences provided in Table S1) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in Table S1), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His (NM_144997.7), pcDNA3.1 TBC1D5HA (NM_014744.2), and pcDNA3.1 GFP-TFEB (NM_007162.2) were bought from GenScript. .. The following plasmids were obtained from Addgene (https://www.addgene.org/): pRK5 Flag-FNIP2 (RRID:Addgene_ 72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) (Tsun et al., 2013); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) (Falcón-Pérez et al., 2005); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_ 99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) (Shen et al., 2017); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) (Mainou and Dermody, 2012); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) (Romero Rosales et al., 2009).

    Article Title: Maspardin/SPG21 controls lysosome motility and TFEB phosphorylation through RAB7 positioning
    Article Snippet: The SPG21 sequence was amplified by PCR using In-Fusion primers (primer sequences provided in ) and inserted using the In-Fusion HD Cloning Kit (Takara Bio) into an EcoRI-digested pcDNA3.1(+) plasmid. .. The c.601insA and c.322 C>G (p.A108P) mutations were introduced into the SPG21 sequence by triple PCR (primer sequences provided in ), and the mutated sequences were then integrated using the In-Fusion HD Cloning Kit (Takara Bio) into the pcDNA3.1(+) plasmid linearized using BamHI and BsiWI restriction sites or EcoRI restriction sites, respectively. pcDNA3.1 Folliculin-His ( NM_144997.7 ), pcDNA3.1 TBC1D5-HA ( NM_014744.2 ), and pcDNA3.1 GFP-TFEB ( NM_007162.2 ) were bought from GenScript. .. The following plasmids were obtained from Addgene ( https://www.addgene.org/ ): pRK5 Flag-FNIP2 (RRID:Addgene_72294) from David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) ( ); LAMP1-mGFP (RRID: Addgene_34831) from Esteban Dell’Angelica (University of California, Los Angeles, California, USA) ( ); pRK5 HA-RagA WT (RRID: Addgene_99710), pRK5 HA-RagA CA Q66L (RRID: Addgene_99712), pRK5 HA-RagC WT (RRID: Addgene_99718), and pRK5 HA-RagC CA S75N (RRID: Addgene_99719) were deposited by David Sabatini (Czech Academy of Sciences, Prague, Czech Republic) and Kuang Shen (Whitehead Institute for Biomedical Research and MIT, Cambridge, Massachusetts, USA) ( ); EGFP-RILP (RRID: Addgene_110498) from Terence Dermody (University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA) and Bernardo Mainou (Emory University School of Medicine, Atlanta, Georgia, USA) ( ); and pGEX-4T-3-RILP (RRID: Addgene_79149) from Aimee Edinger (University of California, Irvine, California, USA) ( ).

    Amplification:

    Article Title: Multiple interactions recruit BLTP2 to ER-PM contacts to control plasma membrane dynamics
    Article Snippet: .. The full BLTP2^EGFP or BLTP2^Halo sequence was further amplified and inserted between the XbaI and EcoRI restriction sites in a pCAG vector using the In-Fusion system (Takara), respectively. ..

    Article Title: A CD25–chemokine receptor complex initiates noncanonical IL-2 signaling
    Article Snippet: .. FLAG-tagged human CCR7 was PCR amplified and cloned into the BamHI and EcoRI restriction sites of the lentiviral pLVX-Het1 vector (Takara Bio; catalog no.: 635075). .. The resulting plasmid was transfected into HEK293T cells using polyethyleneimine (Polysciences; catalog no.: 24765), followed by a 48-h incubation.

    Article Title: Multiple interactions recruit BLTP2 to ER-PM contacts to control plasma membrane dynamics.
    Article Snippet: .. BLTP2 interactions at ER-PM contacts https://doi.org/10.1083/jcb.202504027 D ow nloaded from http://rupress.org/jcb/article-pdf/224/11/e202504027/1949818/jcb_202504027.pdf by Indian Institute O f Technology Bom bay user on 07 Septem ber 2025 was further amplified and inserted between the XbaI and EcoRI restriction sites in a pCAG vector using the In-Fusion system (Takara), respectively. ..

    other:

    Article Title: A scalable screening platform for discovering predominant miRNA target genes and druggable siRNA sequences
    Article Snippet: shGAPDH GIPZ plasmid (pGIPZ) was purchased from Horizon Discovery (cat# RHS4371).



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